Actin is highly conserved and participates in more protein-protein interactions than any other known protein. It is a critical player in many cellular functions, ranging from cell motility to the regulation of transcription. Even though Amoebozoa has been evolutionarily linked as closely related to human, there is little research done related to cytoskeletal proteins in Amoebozoa. Understanding the relationship between actin and its function in various species of Amoebozoa can offer novel information on reproduction, locomotion, and organismal structure. A gene inventory was conducted in various Amoebozoa genomes using human actin and actin-related gene families as a query with implementation of Python scripting and NCBI BLAST. The development of this gene inventory offered a catalog of actin and actin-related gene paralogs in amoebozoa. Furthermore, two actin-related genes (ACTR5 and ACTR8 ) were identified as not present in parasitic amoebozoa. These genes are involved in double-strand break repair and functional organization of mitotic chromosomes, respectively. Phylogenetic analysis of the inventories gene families allowed examination of recent and ancient paralogy in the group. Developments from this research will prompt further investigation into the expression analysis of the detected genes and their role in the life cycle and development of amoebozoa.
